Technical Reference #349

349.

Near-Infrared Fluorescent Rgd Peptides For Optical Imaging Of Integrin Rvâ3 Expression In Living Mice Zhen Cheng, Yun Wu, Zhengming Xiong, Sanjiv Sam Gambhir, and Xiaoyuan Chen, Stanford University, Bioconjugate Chemistry, 16(349), 1435, (2005)
Link To Paper

Abstract:
Near-infrared fluorescence optical imaging is a powerful technique for studying diseases at the molecular level in preclinical models. We recently reported that monomeric RGD peptide c(RGDyK) conjugated to the NIR fluorescent dye specifically targets inte

Keywords:
monomeric RGD peptide of c(RGDyK), tumor, Near-infrared fluorescence (NIRF) optical imaging, cell adhesion molecule integrin Rvâ3,

Materials & Methods:
All commercially available chemical reagents were used without further purification. 9-Fluorenylmethoxycarbonyl (Fmoc) amino acids and 2-chlorotrityl chloride resin were purchased from Novabiochem (San Diego, CA). Arginine and lysine were protected by

Microscopic Technique
Fluorescence Microscopy, Near-infrared fluorescence (NIRF), Optical imaging

Cell Type(s)
U87MG