Technical Reference #349

349.

Near-Infrared Fluorescent Rgd Peptides For Optical Imaging Of Integrin Rvâ3 Expression In Living Mice Zhen Cheng; Yun Wu; Zhengming Xiong; Sanjiv Sam Gambhir; and Xiaoyuan Chen, Stanford University, Bioconjugate Chemistry, 16(349), (2005)
Link To Paper

Abstract:
Near-infrared fluorescence optical imaging is a powerful technique for studying diseases at the molecular level in preclinical models. We recently reported that monomeric RGD peptide c(RGDyK) conjugated to the NIR fluorescent dye specifically targets inte

Keywords:
monomeric RGD peptide of c(RGDyK); tumor; Near-infrared fluorescence (NIRF) optical imaging; cell adhesion molecule integrin Rvâ3;

Materials & Methods:
All commercially available chemical reagents were used without further purification. 9-Fluorenylmethoxycarbonyl (Fmoc) amino acids and 2-chlorotrityl chloride resin were purchased from Novabiochem (San Diego CA). Arginine and lysine were protected by

Microscopic Technique
Fluorescence Microscopy, Near-infrared fluorescence (NIRF), Optical imaging

Cell Type(s)
U87MG