Technical Reference #347

347.

Global Survey Of Organ And Organelle Protein Expression In Mouse: Combined Proteomic And Transcriptomic Profiling Thomas Kislinger; Brian Cox; Anitha Kannan; Clement Chung; Pingzhao Hu; Alexandr Ignatchenko; Michelle S. Scott; Anthony O. Gramolini; Quaid Morris; Michael T. Hallett; Janet Rossant; Timothy R. Hughes; Brendan Frey; and Andrew Emili, University of Toronto, Molecular & Cellular Proteomics, 125(347), (2006)
Link To Paper

Abstract:
A global protein survey is needed to gain systems-level insights into mammalian cell signaling and information flow. Human Jurkat T leukemic cells are one of the most important model systems for T cell signaling study but no comprehensive proteomics surv

Keywords:
mouse; subcellular factionation; transcriptional profiling; proteomic profiles; putative transmembrane helices (TMH);

Materials & Methods:
For these experiments we generated sequence-verified clones for ten representative gene products as N-terminal fusions to a green fluorescent marker protein (GFP) and examined their corresponding localization patterns in transient transfections of HEK

Microscopic Technique
Confocal Microscopy

Cell Type(s)
HEK-293