Technical Reference #346

346.

Raman Imaging Of Floating Cells Caitriona M. Creely; Giovanni Volpe; Gajendra P. Singh; Marta Soler; Dmitri V. Petrov, Institut de Ciencies Fotoniques, Optics Express, 13(346), (2005)
Link To Paper

Abstract:
Raman imaging can yield spatially resolved biochemicalinformation from living cells. To date there have been no Raman imagespublished of cells in suspension because of the problem of immobilisingthem suitably to acquire space-resolved spectra. In th

Keywords:
Brownian motion; Holographic Optical Tweezers (HOT); Fresnel lens

Materials & Methods:
An asymmetric Jurkat cell was trapped using the HOT and scanned back and forth across the Raman excitation beam in a 5×5 step scan (stepsize 3 µm) and a spectrum was taken at each point. To show the advantage of using optical tweezers for Raman spectrosc

Microscopic Technique
Raman Spectroscopy

Cell Type(s)
Jurkat