Technical Reference #343

Glass Bottom Culture Dishes

This study used MatTek product(s):

Citation in paper containing MatTek reference:
poly-D-lysine-coated coverslips (MatTek; Ashland; MA)

343.

Identification Of Plasma Membrane Macro- And Microdomains From Wavelet Analysis Of Fret Microscopy Kobrinsky; E.; Mager ; D.; Bentil ; S.; Murata ; S.; Abernethy D.; and Soldatov ; N. National Institute on Aging; National Institutes of Health; Baltimore; Maryland; and Department of Pathology; Interdisciplinary Graduate School of Medicine and E, National Institutes of Health, Biophysical Journal, 88(343), (2005)
Link To Paper

Abstract:
HIV-1 envelope glycoprotein-mediated fusion is driven by the concerted coalescence of theHIV-1 gp41 N- and C-helical regions which results in the formation of 6-helix bundles. These two regionsare considered prime targets for peptides and antibodies

Materials & Methods:
COS1 cells were grown on poly-D-lysine coated coverslips (MatTek Ashland MA) in DMEM supplemented with 10% fetal calf serum. COS1 cells were transfected with CKAR-pcDNA3 using the Effectene kit (Qiagen Valencia CA)."""

Microscopic Technique
Fluorescence Microscopy, Fluorescence Resonance Energy Transfer (FRET)

Cell Type(s)
COS-1