Technical Reference #340

Glass Bottom Culture Dishes

This study used MatTek product(s):

Citation in paper containing MatTek reference:
35-mm glass bottom microwell dishes (MatTek Corp.)

340.

Dictyostelium Macroautophagy Mutants Vary In The Severity Of Their Developmental Defects Otto; G. P.; Wu; M. Y.; Kazgan; N.; Anderson; O. R.; and Kessin ; R.; Department of Anatomy and Cell Biology; Columbia University; New York; NY and the Department of Biology; Lamont-Doherty Earth Observatory; Columbia University; Palisades; NY. J., Columbia University, The Journal of Biological Chemistry, 279(340), (2004)
Link To Paper

Abstract:
Macroautophagy is the major mechanism that eukaryotes use to recycle cellular components during stressful conditions. We have shown previously that the Atg12-Atg5 conjugation system required for autophagosome formation in yeast is necessary for Dictyost

Materials & Methods:
To examine the localization of GFP fusion proteins axenic cells were incubated overnight in HL5 medium supplemented with 10 microg/ml G418 in 35-mm glass bottom microwell dishes (MatTek Corp.).

Microscopic Technique
Fluorescence Microscopy

Cell Type(s)
Axenic