Technical Reference #339

Glass Bottom Culture Dishes

This study used MatTek product(s):

Citation in paper containing MatTek reference:
35-mm glass-bottomed culture dishes (poly-D-lysine coated; MatTek; Ashland)

339.

Spatial And Temporal Cellular Responses To Single-Strand Breaks In Human Cells Okano; S.; Lan; L.; Caldecott; KW.; Mori; T.; Yasui; A. Department of Molecular Genetics; Institute of Development; Aging and Cancer; Tohoku University; Sendai; Radioisotope Research Center; Nara Medical University; Nara; Japan; Genome Damage an, Tohoku University, Molecular and Cellular Biology, 23(339), (2003)
Link To Paper

Abstract:
DNA single-strand breaks (SSB) are one of the most frequent DNA lesions produced by reactive oxygen species and during DNA metabolism but the analysis of cellular responses to SSB remains difficult due to the lack of an experimental method to produce SSB

Materials & Methods:
Cell monolayers in 35-mm glass-bottomed culture dishes (poly-d-lysine coated; MatTek Ashland Oreg.) were covered with a polycarbonate isopore membrane filter with pores of 3 µm in diameter (Millipore) and UV irradiated with a germicidal lamp (GL-10; Tos

Microscopic Technique
Fluorescence Microscopy

Cell Type(s)
XPA-UVDE