Technical Reference #337

337.

Early Molecular Events In The Assembly Of The Focal Adhesion-Stress Fiber Complex During Fibroblast Spreading Zimerman; B.; Volberg; T.; and Geiger; B. Department of Molecular Cell Biology; The Weizmann Institute of Science; Rehovot; Israel. Cell Motility and the Cytoskeleton 58; (); 4-59; (004)., The Weizmann Institute of Science, Cell Motility and the Cytoskeleton, 58(337), (2004)
Link To Paper

Abstract:
Cell adhesion to the extracellular matrix triggers the formation of integrin-mediated contact and reorganization of the actin cytoskeleton. Examination of nascent adhesions formed during early stages of fibroblast spreading reveals a variety of forms of

Materials & Methods:
After the electroporation the cells were collected by centrifugation resuspended in DMEM and plated on glass-bottom microwell dishes (MatTek Ashlend MA)

Microscopic Technique
Video Microscopy, Time Lapse, Scanning Electron Microscopy, Immunofluorescence, Digital Immunofluorescence Microscopy

Cell Type(s)
REF52