Technical Reference #334

Glass Bottom Culture Dishes

This study used MatTek product(s):

Citation in paper containing MatTek reference:
glass-bottomed dishes (MatTek Corp.; Ashland; MA)

334.

Frequency-Domain Fluorescence Microscopy With The Led As A Light Source Herman; P.; Maliwal; BP.; Lin; HJ.; Lakowicz; JR. University of Maryland School of Medicine; Center for Fluorescence Spectroscopy; Department of Biochemistry and Molecular Biology; Baltimore; MD; USA. Journal Microscopy; 0; ; 76-0; 76-8; (00), University of Maryland, Journal of Microscopy, 203(334), (2001)
Link To Paper

Abstract:
We describe a frequency-domain lifetime fluorometer basedon a microscope and a modulated light-emitting diode (LED)excitation source (370/460 nm) which operates in thefrequency range 120 Hz±250 MHz. We collected multi-frequency phase and modulation fluor

Keywords:
Fluorescence; frequency-domain; LED; lifetime; microscopy

Materials & Methods:
Mouse fibroblasts (3T3 Swiss albino ATCC number CCL-92) were grown at 37 Celcius and a 5% CO2 atmosphere in galssbottomed disehs (MatTek Corp. Ashland MA) containing Dulbecco's modified Eagle's medium with 10% calf serum.

Microscopic Technique
Fluorescence Microscopy, Inverted Epifluorescence Microscopy

Cell Type(s)
3T3