Technical Reference #333

333.

Human Lung Cancer Cells Express Functionally Active Toll-Like Receptor 9 Droemann; D.; Albrecht; ; D.; Gerdes; J.; Ulmer; AJ.; Branscheid; D.; Vollmer4; E.; Dalhoff5; K.; Zabel;5 ; P.; Goldmann4; T. Medical Clinic; Research Center Borstel; Borstel; Germany; Department of Immunology and Cell Biology; Research Center, Research Center Borstel, Respiratory Research, 6(333), (2005)
Link To Paper

Abstract:
Here we show that TLR9 is expressed in a selection of human lung cancer tissues and various tumor cell lines. The expression of functionally active TLR9 in human malignant tumors might affect treatment approaches using CpG-ODN and shows that malignant cel

Materials & Methods:
A549-cells were seeded in 35-mm glass bottom dishes (MatTek Corp.) overnight. Cells were transfected with GFP-huTLR9 using Polyfect (Qiagen) according to the manufacturer's instructions or incubated in medium.

Microscopic Technique
Confocal Microscopy

Cell Type(s)
A549