Technical Reference #332

332.

Polarized Expression Of Members Of The Solute Carrier Slc19A Gene Family Of Water-Soluble Multivitamin Transporters: Implications For Physiological Function Boulware; MJ.; Subramanian; VS.; Said; HM; Marchant; JS. Department of Pharmacology; University of Minnesota Medical School; MN; U.S.A.; Department of Medicine and Physiology; University of California; Irvine; CA; U.S.A. and VA Medical Center; Long, University of Minnesota, Biochemical Journal, 376(332), (2003)
Link To Paper

Abstract:
Humans lack biochemical pathways for the synthesis of the micro-nutrients thiamine and folate. Cellular requirements are met through membrane transport activity which is mediated by proteins of the SLC19A gene family. By using live-cell confocal imaging

Keywords:
folate; polarity; thiamine; transport; vitamin

Materials & Methods:
For transient transfections on cover-glasses cells were grown to 90% confluency on sterile glass-bottomed Petri dishes (MatTek MA U.S.A.) and incubated with 1 mg of plasmid DNA and LIPOFECTAMINETM 2000 in OPTIMEM (Invitrogen).

Microscopic Technique
Confocal Microscopy

Cell Type(s)
MDCK