Technical Reference #329

329.

E5 Protein Of Human Papillomavirus Type 16 Protects Human Foreskin Keratinocytes From Uv B-Irradiation-Induced Apoptosis Zhang;; B.; Spandau; D.; and Roman; A.; Department of Microbiology and Immunology; Indiana University School of Medicine; and Walther Cancer Institute; Indianapolis; IN; Departments of Dermatology and Biochemistry and Molecular Biology. Journal, Indiana University, The Journal of Virology, 76(329), (2002)
Link To Paper

Abstract:
The human papillomavirus type 16 (HPV16) E5 protein associates with the epidermal growth factor receptor (EGFR) and enhances the activation of the EGFR after stimulation by EGF in human keratinocytes. Phosphatidylinositol 3-kinase (PI3K) and ERK1/2 mitoge

Keywords:
HPV16; UV B irradiation; TUNEL assay; Northern blotting; retrovirus infection

Materials & Methods:
Infected HFKs (105) were grown on 35-mm-diameter glass-bottomed dishes (MatTek Corporation) coated with type 1 collagen (Sigma).

Microscopic Technique
Fluorescence Microscopy

Cell Type(s)
HFK