Technical Reference #326

Glass Bottom Culture Dishes

This study used MatTek product(s):

Citation in paper containing MatTek reference:
MatTek dishes previously coated with poly-L-lysine

326.

Planar And Vertical Signals Control Cellular Differentiation And Patterning In The Mammalian Cochlea Montcouquiol; M.; and Kelley; MW. Section on Developmental Neuroscience; National Institute on Deafness and Other Communication Disorders-National Institutes of Health; Rockville; MD; USA. The Journal of Neuroscience; ; (8); 9469-9478; (00)., National Institutes of Health, The Journal of Neuroscience, 23(326), (2003)
Link To Paper

Abstract:
The sensory epithelium of the mammalian cochlea is composed of a regular mosaic of sensory hair cells and nonsensory supporting cells. During development differentiation occurs in a gradient that progresses along the axis of the cochlea from base to apex

Keywords:
cochlea; mesenchyme; development; hair cell; inner ear; epithelium

Materials & Methods:
After dissection the developing sensory epithelium and associated mesenchymal cells were established in an individual MatTek dish (MatTek Corporation Ashland MA) as an intact explant culture. For some cultures the apical 20-25% of the developing epith

Microscopic Technique
Fluorescence Microscopy

Cell Type(s)
mesenchymal