Technical Reference #322

322.

Visualizing The Site And Dynamics Of Igg Salvage By The Mhc Class I-Related Receptor; Fcrn Ober; RJ.; Martinez; C.; Vaccaro; C.; Zhou; J.; Ward; ES. Cancer Immunobiology Center and Center for Immunology; University of Texas Southwestern Medical Center; Dallas; TX ; USA. The Journal of Immunology; 7; (4); 0-09; (004)., University of Texas, The Journal of Immunology, 172(322), (2004)
Link To Paper

Abstract:
The MHC class I-related receptor FcRn plays a central role in regulating the serum levels of IgG. FcRn is expressed in endothelial cells suggesting that these cells may be involved in maintaining IgG levels. We have used live cell imaging of FcRn-green

Materials & Methods:
"HMEC-1 cells were transiently transfected with FcRn-GFP and 2m expression constructs (2–3 µg of each) using Nucleofector technology (Amaxa Biosystems Cologne Germany) and the protocol recommended for the transfection of human endothelial cells. Immedia

Microscopic Technique
Fluorescence Microscopy

Cell Type(s)
HMEC-1