Technical Reference #320

320.

Mechanical Properties Of Individual Focal Adhesions Probed With A Magnetic Microneedle Matthews; ; BD.; Overby; DR.; Alenghatm; FJ.; Karavitis; J.; Numaguchi; Y.; Allen; PG.; Ingber; DE. Vascular Biology Program; Department of Pathology; Children's Hospital; Harvard Medical School; Boston; MA; USA. Department of Pediatrics; Mas, Children's Hospital Boston, Biochemical and Biophysical Research Communications, 313(320), (2004)
Link To Paper

Abstract:
A permanent magnetic microneedle was developed to apply tensional forces to integrin receptors via ligand-coated magneticmicrobeads while optically analyzing the mechanical properties of individual focal adhesions. Force application (130pN for 3 s)thr

Keywords:
Mechanical stress; Integrins; Focal adhesion; Mechanotransduction; Cell mechanics; Magnetometry

Materials & Methods:
Cells were cultured in DMEM supplemented with low (0.5%) FCS for 24 h trypsinized (Trypsin–EDTA Gibco) collected and then seeded (1.5104 cells/dish) onto glass-bottomed 35mm dishes (MatTek) that were pre-coated with 500 ng/cm2 fibronectin.

Microscopic Technique
Optical Microscopy, Real Time, Fluorescence Microscopy

Cell Type(s)
H4