Technical Reference #319

319.

Centrosome Maturation: Measurement Of Microtubule Nucleation Throughout The Cell Cycle By Using Gfp-Tagged Eb1 Piehl; M.; Tulu; US; Wadsworth; P.; Cassimeris; L. Department of Biological Sciences; Lehigh University; Bethlehem; PA; USA. and Department of Biology; University of Massachusetts; Amherst; MA; USA. Proc Natl Acad Science; 0; (6); 584-588; (0, Lehigh University, PNAS, 101(319), (2004)
Link To Paper

Abstract:
Understanding how cells regulate microtubule nucleation during the cell cycle has been limited by the inability to directly observe nucleation from the centrosome. To view nucleation in living cells we imaged GFP-tagged EB1 a microtubule tip-binding pro

Materials & Methods:
Coverslips for live cell imaging were placed in Rose chambers (9) or grown directly on glass-bottom culture dishes (MatTek Ashland MA).

Microscopic Technique
Confocal Microscopy, Inverted confocal laser scanning

Cell Type(s)
LLC-PK