Technical Reference #311

311.

Visualizing Telomere Dynamics In Living Mammalian Cells Using PNA Probes Molenaar; C.; Wiesmeijer; K.; Verwoerd; N.; Khazen; S.; Eils; R.; Tanke; H.; and Dirks; R. Department of Molecular Cell Biology; Leiden University Medical Center; Leiden; The Netherlands and Intelligent Bioinformatics Systems; das Deutsche Krebsforsc, Leiden University, The EMBO Journal, 22(311), (2003)
Link To Paper

Abstract:
Chromosome ends are protected from degradation by the presence of the highly repetitive hexanucleotide sequence of TTAGGG and associated proteins. These so-called telomeric complexes are suggested to play an important role in establishing a functional nuc

Keywords:
ALT; live cell imaging; PML; PNA; telomeres

Materials & Methods:
U2OS cells (ATCC HTB-96) HeLa and MS5 mouse cells were grown on coverslips in 3.5-cm Petri dishes (MatTek Corp.) in RPMI 1640 without phenol red supplemented with 5% fetal calf serum and buffered with 25 mM HEPES buffer to pH 7.2 (Gibco-BRL).

Microscopic Technique
Fluorescence Microscopy, Live Cell

Cell Type(s)
U2OS