Technical Reference #303

303.

Electroporation Loading And Photoactivation Of Caged Insp3: Tools To Investigate The Relation Between Cellular Atp Release In Response To Intracellular Insp3 Elevation Braet, K., Mabilde, C., Cabooter, L., Rapp, G., Leybaert, L. Physiology and Pathophysiology, Ghent University, Ghent, Belgium. Rapp OptoElectronic GmbH, Hamburg, Germany. Journal of Neuroscience Methods, , (), 8-89, (004)., Ghent University, The Journal of Neuroscience Methods, 132(303), 82, (2004)
Link To Paper

Abstract:
Photolytic liberation of InsP3 in single cells triggers cell-to-cell propagating calcium changes that are communicated by a gap junctional and a paracrine purinergic pathway involving InsP3-triggered ATP release. We investigated the relation between the I

Keywords:
Flash photolysis; Electroporation; Caged compounds; InsP3; ATP release; Calcium signaling; Calcium waves

Materials & Methods:
...this study we used ECV304 cells (European Collection of Animal Cell Cultures, Salisbury, UK) grown on glass bottom microwells (MatTek Corporation, Ashwood, MA, USA). Cultures were maintained in Medium-199 (Gibco, Merelbeke, Belgium) with 10% fetal bovi

Microscopic Technique
z-none specified

Cell Type(s)
ECV304