Technical Reference #301

301.

Efficient Active Transport Of Gene Nanocarriers To The Cell Nucleus Suh; J.; Wirtz; ; D.; and Hanes; J.; Departments of Biomedical Engineering; Chemical and Biomolecular Engineering; and Materials Science and Engineering; Molecular Biophysics Program; The Johns Hopkins University; Baltimore; MD; USA. PNAS; 00, Johns Hopkins University, PNAS, 100(301), (2003)
Link To Paper

Abstract:
The intracellular transport of therapeutic gene carriers is poorly understood limiting the rational design of efficient new vectors. We used live-cell real-time multiple particle tracking to quantify the intracellular transport of hundreds of individual

Materials & Methods:
COS-7 cells were plated onto glass-bottom tissue culture plates (MatTek Ashland MA).

Microscopic Technique
Fluorescence Microscopy

Cell Type(s)
COS-7