Technical Reference #265

Glass Bottom Culture Dishes

This study used MatTek product(s):

Citation in paper containing MatTek reference:
plastic Petri dish with a cover glass bottom (MatTek Corp.; Ashland; MA)

265.

Increased Birefringence In The Meiotic Spindle Provides A New Marker For The Onset Of Activation In Living Oocytes Liua; L.; Trimarchib; J. R.; Oldenbourgb; R.; Keefea; D.L., Brown University, Biology of Reproduction, 63(265), (2000)
Link To Paper

Abstract:
The newly developed Pol-Scope allows imaging of spindle retardance which is an optical property of organized macromolecular structures that can be observed in living cells without fixation or staining. Experiments were undertaken to examine changes in me

Keywords:
developmental biology; fertilization; IVF/ART; oocyte development; ovum

Materials & Methods:
Oocytes were imaged at 37°C in HKSOM in a plastic Petri dish with a cover glass bottom (MatTek Corp. Ashland MA) or in a custom-fabricated cover-glass-bottom chamber.

Microscopic Technique
Inverted Microscopy

Cell Type(s)
Oocytes