Technical Reference #264

Glass Bottom Culture Dishes

This study used MatTek product(s):

Citation in paper containing MatTek reference:
poly-D-lysine-coated glass-bottom dishes (MatTek Corp., Ashland, MA)

264.

Haploidy But Not Parthenogenetic Activation Leads To Increased Incidence Of Apoptosis In Mouse Embryos Liu, L., Trimarchib, J. R., Keefea, D.L. aDepartment of Obstetrics and Gynecology, Women and Infants Hospital, Brown University, Providence, RI, bLaboratory for Reproductive Medicine, Marine Biological Laboratory, Woods Hole, MA, Biology of Reproduction,, Brown University, Biology of Reproduction, 66, 205, (2001)
Link To Paper

Abstract:
Aneuploidy underlies failed development and possibly apoptosis of some preimplantation embryos. We employed a haploid model in the mouse to study the effects of aneuploidy on apoptosis in preimplantation embryos. Mouse metaphase II oocytes that were activ

Keywords:
apoptosis, developmental biology, early development, embryo, ovum

Materials & Methods:
Fura-loaded oocytes were washed extensively with KSOM and transferred to poly-D-lysine-coated glass-bottom dishes (MatTek Corp., Ashland, MA);Collection of Mouse Oocytes and In Vitro Culture; IVF and Parthenogenetic Activation of Oocytes; Chromosomal Analysis; Detection of Apoptosis by TUNEL Assay; Measurement of Cytosolic Ca2+

Microscopic Technique
Fluorescence Microscopy

Cell Type(s)
Oocytes