Technical Reference #262

262.

Imaging Of Procollagen Transport Reveals Copi-Dependent Cargo Sorting During Er To-Golgi Transport In Mammalian Cells Stephens; D.J.; and Pepperkok; R., European Molecular Biology Laboratory, The Journal of Cell Science, 115(262), (2002)
Link To Paper

Abstract:
We have examined the ER-to-Golgi transport of procollagen which when assembled in the lumen of the ER is thought to be physically too large to fit in classically described 60-80 nm COPI- and COPII-coated transport vesicles. We found that procollagen ex

Keywords:
Procollagen; ER; COPII; Golgi; sorting

Materials & Methods:
Cells were plated 24-48 hours prior to injection on live cell dishes (MatTek Ashland MA USA)

Microscopic Technique
Fluorescence Microscopy, Immunofluorescence

Cell Type(s)
Vero