Technical Reference #260

Glass Bottom Culture Dishes

This study used MatTek product(s):

Citation in paper containing MatTek reference:
Sterile glass-bottom; poly-D-lysine-coated dishes (MatTek Corp.; Ashland; MA)

260.

Phosphoinositide Binding By The Pleckstrin Homology Domains Of Ipl And Tih 1 Saxena; A.; Morozov; P.; Frank; D.; Musalo; R.; Lemmon; M.A.; Skolnik4; E.Y.; and Tycko; B.;, Columbia University, The Journal of Biological Chemistry, 277(260), (2002)
Link To Paper

Abstract:
The Ipl protein consists of a single pleckstrin homology (PH) domain with short N- and C-terminal extensions. This protein is highly conserved among vertebrates and it acts to limit placental growth in mice. However its biochemical function is unknown.

Materials & Methods:
Sterile glass-bottom poly-D-lysine-coated dishes (MatTek Corp. Ashland MA) were used for translocation assays in COS7 cells.

Microscopic Technique
Fluorescence Microscopy, Inverted Fluorescence Microscopy

Cell Type(s)
COS-7