Technical Reference #255

255.

Direct Evidence Of Active And Rapid Nuclear Degradation Triggered By Vacuole Rupture During Programmed Cell Death In Zinnia Obara; K.; Kuriyama; H.; and Fukuda; H.; Department of Biological Sciences; Graduate School of Science; University of Tokyo; Tokyo; Japan. Plant Physiology; (5); 65-66; (00)., University of Tokyo, Plant Physiology, 125(255), (2001)
Link To Paper

Abstract:
Differentiation into a tracheary element (TE) is a typical example of programmed cell death (PCD) in the developmental processes of vascular plants. In the PCD process the TE degrades its cellular contents and becomes a hollow corpse that serves as a wate

Materials & Methods:
After stained cells were transferred onto poly-d-Lyscoated dishes (Glass Bottom Microwell Dishes MatTek Corporation Ashland MA) they were placed on the inverted platform of a confocal laser scanning microscope (Meridian Instruments Far East Tokyo).

Microscopic Technique
Confocal Microscopy

Cell Type(s)
mesophyll