Technical Reference #254

254.

Apical And Basolateral Endocytic Pathways Of Mdck Cells Meet In Acidic Common Endosomes Distinct From A Nearly-Neutral Apical Recycling Endosome Wanga, E., Browna, P.S., Aroetic, B., Chapinb, S.J., Mostovb, K.E., and Dunna, K.W., a Department of Medicine, Indiana University School of Medicine, Indianapolis, IN, USA b Department of Anatomy and Department of Biochemistry and Biophysics, University o, Indiana University, Traffic, 1(6), 491, (2000)
Link To Paper

Abstract:
Quantitative confocal microscopic analyses of living, polarized MDCK cells demonstrate different pH profiles for apical and basolateral endocytic pathways, despite a rapid and extensive intersection between the two. Three-dimensional characterizations of

Keywords:
acidification, endocytosis, endosome, epithelia, immunoglobulin A, MDCK, polarity, polymeric Ig receptor, transferring, transcytosis

Materials & Methods:
After removing the legs of the filter units, living or fixed cells were observed by placing the entire filter unit on two 50µ m tape spacers attached to the coverslip of a coverslip-bottomed 35 mm dish (Mattek, Ashland, MA) mounted on the stage of an inve

Microscopic Technique
Confocal Microscopy

Cell Type(s)
MDCK