Technical Reference #254

254.

Apical And Basolateral Endocytic Pathways Of Mdck Cells Meet In Acidic Common Endosomes Distinct From A Nearly-Neutral Apical Recycling Endosome Wanga; E.; Browna; P.S.; Aroetic; B.; Chapinb; S.J.; Mostovb; K.E.; and Dunna; K.W.; a Department of Medicine; Indiana University School of Medicine; Indianapolis; IN; USA b Department of Anatomy and Department of Biochemistry and Biophysics; University o, Indiana University, Traffic, 1(254), (2000)
Link To Paper

Abstract:
Quantitative confocal microscopic analyses of living polarized MDCK cells demonstrate different pH profiles for apical and basolateral endocytic pathways despite a rapid and extensive intersection between the two. Three-dimensional characterizations of

Keywords:
acidification; endocytosis; endosome; epithelia; immunoglobulin A; MDCK; polarity; polymeric Ig receptor; transferring; transcytosis

Materials & Methods:
After removing the legs of the filter units living or fixed cells were observed by placing the entire filter unit on two 50µ m tape spacers attached to the coverslip of a coverslip-bottomed 35 mm dish (Mattek Ashland MA) mounted on the stage of an inve

Microscopic Technique
Confocal Microscopy

Cell Type(s)
MDCK