Technical Reference #251

251.

Live Imaging Of Endogenous Rna Reveals A Diffusion And Entrapment Mechanism For Nanos Mrna Localization In Drosophila Forrest; K.M.; and Gavis; E.R.; Department of Molecular Biology; Princeton University; Princeton; NJ; USA. Current Biology; ; (4-5); 59-68; (00), Princeton University, Current Biology, 13(251), (2003)
Link To Paper

Abstract:
Background: Localization of nanos mRNA to the posterior pole of the Drosophila embryo directs local synthesis of Nanos protein that is essential for patterning of the anterior-posterior body axis and germ cell function. While nanos RNA is synthesized by t

Materials & Methods:
An individual egg chamber was then mounted on an uncoated #1.5 glass bottom culture dish (MatTek) in 200 µl Schneider's medium. Slight back pressure was applied by laying a 4 mm2 square #1 glass coverslip (Corning) on top of the egg chamber with Dumont #5

Microscopic Technique
Confocal Microscopy

Cell Type(s)
Egg chambers