Technical Reference #250

250.

Membrane-Permeant Chelators Can Attenuate Zn2+-Induced Cortical Neuronal Death Canzoniero; L.MT.; Manzerra; P.; Sheline; C.T.; and Choi; D.W.; Washington University School of Medicine; St Louis; MO; USA Neuropharmacology; 45; (); 40-48 (00), Washington University, Neuropharmacology, 45(250), (2003)

Abstract:
Chelating extracellular Zn2+ with the membrane-impermeant Zn2+ chelator CaEDTA can inhibit toxic Zn2+ influx and subsequent neuronal death. However this drug does not cross the blood–brain barrier. In the present study we explored the ability of two m

Keywords:
Apoptosis; Neuroprotection; Toxicity; Intracellular zinc

Materials & Methods:
Neurons for intracellular Zn2+ imaging experiments were prepared similarly and plated on 35-mm glass-bottom dishes (MatTek Ashland MA) coated with poly-D-lysine:laminin (100 ng/ml: 4 ng/ml).

Microscopic Technique
Phase Contrast Microscopy

Cell Type(s)
Mixed cortical cultures