Technical Reference #249

Glass Bottom Culture Dishes

This study used MatTek product(s):

Citation in paper containing MatTek reference:
poly-lysine coated glass-bottom dish (35 mm; MatTek Co.; Ashland; MA; USA)

249.

Ganglioside/Calmodulin Kinase Ii Signal Inducing Cdc42-Mediated Neuronal Actin Reorganization Chen; b; N.; Furuyac; S.; Doib; H.; Hashimotob; Y.; Kudd; Y.; and Higashia;a; b; H.; a Mitsubishi Kagaku Institute of Life Sciences; Tokyo; Japan b Glyco-Chain Functions Laboratory; RIKEN Frontier Research System; Saitama; Japan c Neuronal Circuit Mecha, Institute of Life Sciences, Neuroscience, 120(249), (2003)
Link To Paper

Abstract:
Cell surface glycoconjugates are thought to mediate cell–cell recognition and play roles in neuronal development and functions. We demonstrated here that exposure of neuronal cells to nanomolar levels of gangliosides Neu5Ac8Neu5Ac3Galβ4GlcCer Galβ3GalNAc

Keywords:
CaM-kinase II; filopodia; oligosaccharide; NG108-15 cell; hippocampal neuron; Purkinje cell

Materials & Methods:
Cells cultured on poly-lysine coated glass-bottom dish (35 mm MatTek Co. Ashland MA USA).

Microscopic Technique
Confocal Microscopy

Cell Type(s)
hippocampal