Technical Reference #246

246.

Hepatitis B Virus Enhances Tumor Necrosis Factor-Related Apoptosis-Inducing Ligand (Trail) Cytotoxicity By Increasing Trail-R1/Death Receptor 4 Expression Janssen; ; H.LA.; Higuchi; H.; Abdulkarim; A.; and Gores; G.J; Department of Gastroenterology and Hepatology; Erasmus Medical Center Rotterdam; Rotterdam; The Netherlands Division of Gastroenterology and Hepatology; Mayo Medical School; Clinic and, Erasmus Medical Center Rotterdam, Journal of Hepatology, 39(246), (2003)

Abstract:
Background/Aims: Apoptosis by death receptors such as Fas and tumor necrosis factor (TNF)-alpha receptor-1 play a significant role in the pathogenesis of hepatitis B virus (HBV)-infections. Although liver also expresses death receptors for TNF-related a

Keywords:
Apoptosis; Lamivudine

Materials & Methods:
Cells were cultured on 35 mm glass bottom microwell dishes (Mattek Corp. Ashland MA) at a density of 1000 cells/dish. Intracellular total caspase activity was measured in single cells using the fluorophore tagged pancaspase inhibitor carboxyfluorescein-

Microscopic Technique
Fluorescence Microscopy, Inverted Fluorescence Microscopy

Cell Type(s)
HEP