Technical Reference #245

Glass Bottom Culture Dishes

This study used MatTek product(s):

Citation in paper containing MatTek reference:
35-mm tissue culture dishes with a glassbottomed microwell (P35G-1.5-14-C; No. 1.5; uncoated; MatTek Corporation; Ashland; MA)

245.

Recombinant Apoptin Multimers Kill Tumor Cells But Are Nontoxic And Epitope-Shielded In A Normal-Cell-Specific Fashion Zhanga; YH.; Leliveldb; S.R.; Kooistraa; K.; Molenaarc; C.; Rohna; J.L.; Tanke; H.J.; Abrahamsb; J.P.; and Noteborna; c; MHM.; a Leadd BV; Leiden; The Netherlands b Department of Chemistry; Leiden University; Leiden; The Netherlands c Department of Mol, Leiden University, Experimental Cell Research, 289(245), (2003)
Link To Paper

Abstract:
Recombinant Apoptin multimers kill tumor cells but are nontoxic and epitope-shielded in a normal-cell-specific fashion.

Keywords:
Apoptin; Apoptosis; Epitope shielding; Multimers; Normal-specific processes; Tumor-specific therapy

Materials & Methods:
For microinjection cells were seeded in 35-mm tissue culture dishes with a glass-bottomed microwell (P35G-1.5-14-C No. 1.5 uncoated MatTek Corporation Ashland MA) at 30–40% confluence 24 h prior to microinjection.

Microscopic Technique
Fluorescence Microscopy, Microinjection

Cell Type(s)
Saos-2