Technical Reference #244

244.

Regulation Of Cytosolic Phospholipase A2 Translocation John H. Evans; Daniel J. Fergus; Christina C. Leslie, National Jewish Medical and Research Center, Advances in Enzyme Regulation, 43(244), (2003)

Abstract:
The hormonally regulated Ca(2+)-dependent enzyme cytosolic phospholipase A(2) (cPLA(2)) is activated by a range of inflammatory stimuli. Tumour necrosis factor-alpha (TNF) is one of the first known stimuli for cPLA(2) but it is not known whether both TNF

Keywords:
Phospholipase A2; Phospholipid; Membrane-binding; Arachidonic acid; Calcium; Phosphorylation; Eicosanoid; Prostaglandin; Leukotrienes; 5-lipoxygenase; Cyclooxygenase; Inflammation; Golgi; Endoplasmic reticulum; Nuclear envelope; Nucleus; Mitogen-activated

Materials & Methods:
MDCK cells were grown on glass-bottomed 35 mm tissue culture dishes (MatTek) (1×104 cells/cm2) transfected with the fusion constructs and incubated in serum-free DMEM overnight to quiesce the cells.

Microscopic Technique
Confocal Microscopy, Confocal laser scanning fluorescence microscopy

Cell Type(s)
MDCK