Technical Reference #243

243.

Spatiotemporal Laser Inactivation Of Inositol 1;4;5-Trisphosphate Receptors Using Synthetic Small-Molecule Probes Takanari Inoue;1 Kazuya Kikuchi;1;3 Kenzo Hirose; Masamitsu Iino;2 and Tetsuo Nagano1, University of Tokyo, Chemistry & Biology, 10(243), (2003)
Link To Paper

Abstract:
A malachite green-conjugated inositol 145-trisphosphate (MGIP3) induces specific inactivation of IP3 receptor (IP3R) in tissue samples upon laser irradiation. To verify potential usefulness of the method for studies of cellular Ca2+ signaling we conduc

Materials & Methods:
To induce differentiation 50 ng/ml 7S nerve growth factor (NGF Invitrogen) in complete culture medium with a serum level reduced to one-eighth was applied to PC12 cells fixed on a poly-L-lysine-coated glass-bottom dish (MatTek).

Microscopic Technique
Fluorescence Microscopy, Inverted Fluorescence Microscopy

Cell Type(s)
PC12