Technical Reference #242

Glass Bottom Culture Dishes

This study used MatTek product(s):

Citation in paper containing MatTek reference:
35-mm glass-bottom culture dishes coated with poly-L-lysine (MatTek; Ashland; MA)

242.

Increase In Ip3 And Intracellular Ca2+ Induced By Phosphate Depletion In Llc-Pk1 Cells Yutaka Taketani; Mayumi Nomoto; Hironori Yamamoto; Masashi Isshiki; Kyoko Morita; Hidekazu Arai; Ken-ichi Miyamoto; Shigeaki Kato and Eiji Takeda, University of Tokushima, Biochemical and Biophysical Research Communications, 305(242), (2003)
Link To Paper

Abstract:
The mechanisms by which Pi depletion rapidly regulates gene expression and cellular function have not been clarified. Here we found a rapid increase in intracellular ionized calcium [Ca2+]i by phosphate depletion in LLC-PK1 cells using confocal microscop

Keywords:
Inorganic phosphate; Ca2+; IP3; Kidney; LLC-PK1 cells

Materials & Methods:
The cells were transferred 1 day after transfection to 35-mm glass-bottom culture dishes coated with poly-L-lysine (MatTek Ashland MA) and grown for 2 days before use.

Microscopic Technique
Fluorescence Microscopy, Fluorescence Resonance Energy Transfer (FRET)

Cell Type(s)
LLC-PK