Technical Reference #241

241.

Bdnf And Cntf Regulate Cholinergic Properties Of Sympathetic Neurons Through Independent Mechanisms; Molecular And Cellular Neuroscience Slonimsky; J.D.; Yang; B.; Hinterneder; J.M.; Nokes; E.B.; and Birren; S.B.; Department of Biology; Volen Center for Complex Systems; Brandeis University; Waltham; MA; USA. Molecular and Cellular Neuroscience; (); 4; 648-660; (00)., Brandeis University, Molecular and Cellular Neuroscience, 23(241), (2003)

Abstract:
Cultured neonatal sympathetic neurons can synthesize and corelease norepinephrine (NE) and acetylcholine (ACh). Evoked release of NE has an excitatory effect on the beat rate of cocultured cardiac myocytes while ACh release results in myocyte inhibition.

Materials & Methods:
Cultures were grown on glass-bottomed dishes (MatTek Ashland MA) maintained in culture for 3 or 4 days or 3 weeks and then fixed for 10 min in 4% paraformaldehyde.

Microscopic Technique
Fluorescence Microscopy, Inverted Fluorescence Microscopy

Cell Type(s)
HEK-293