Technical Reference #239

239.

Saturated Free Fatty Acids; Palmitic Acid And Stearic Acid; Induce Apoptosis By Stimulation Of Ceramide Generation In Rat Testicular Leydig Cell Lua; ZH.; Mua; YM.; Wanga; BA.; Lib; XL.; Lua; JM.; Lia; JY.; Pana; CY.; Yanasec; T.; and Nawatac; H.; a Department of Endocrinology; Chinese PLA General Hospital; Beijing; PR China; b Department of Central Laboratory; Dalian Municipal Central Hospital;, Chinese PLA General Hospital, Biochemical and Biophysical Research Communications, 303(239), (2003)
Link To Paper

Abstract:
In men obesity has generally been associated with reduced plasma testosterone levels and with elevation of the plasma free fatty acids (FFAs). In this study we investigated the effects of saturated FFAs including palmitic acid (PA) and stearic acid (SA)

Keywords:
Leydig cell; Free fatty acid; Palmitic acid; Stearic acid; Arachidonic acid; Apoptosis; Ceramide; Bcl-2; Bax; Fumonisin B1

Materials & Methods:
One day before treatment the cells were divided into 35-mm glass-bottom dishes (MatTek Ashland MA) and were then treated with 400 µM PA for 48 h.

Microscopic Technique
Fluorescence Microscopy

Cell Type(s)
LC-540