Technical Reference #238

238.

Expression And Functional Characterisation Of A Human Chimeric Nicotinic Receptor With [Alpha]6[Beta]4 Properties Evans; N.M.; Bose; S.; Benedetti; G.; Zwart; R.; Pearson; K.H.; McPhie; G.I.; Craig; P.J.; Benton; J.P.; Volsen; S.G.; Sher; E.; and Broad L.M.; Eli Lilly and Company Ltd.; Lilly Research Centre; Erl Wood Manor; Windlesham; Surrey; UK. European Journal of, Lilly Research, European Journal of Pharmacology, 466(238), (2003)

Abstract:
Despite being cloned several years ago the expression of functional nicotinic acetylcholine receptors containing the human 6 subunit in recombinant mammalian cell lines has yet to be demonstrated. The resulting lack of selective ligands has hindered the

Keywords:
Nicotinic receptor; a6 Subunit; Chimera; HEK-293 cell; (Human)

Materials & Methods:
Transiently transfected cells plated on poly-d-lysine-coated glass bottomed 35 mm micro-well dishes (MatTEK MA USA) were loaded with 2 M Fura-2- acetoxymethylester (AM) diluted in growth medium at 37 °C for 30 min.

Microscopic Technique
Fluorescence Microscopy, Inverted Epifluorescence Microscopy

Cell Type(s)
HEK-293