Technical Reference #234

234.

In Vivo Activation Of Pro-Form Bombyx Cysteine Protease (Bcp) In Silkmoth Eggs: Localization Of Yolk Proteins And Bcp; And Acidification Of Yolk Granules Yamahama; Y.; a; Uto; N.;a; Tamotsu S.;b; T. Miyatac; Yamamoto; Y.d; Watabe; S.e and Takahashi; S. Y. d; a Department of Biology; Hamamatsu University; Hamamatsu; Japan; b Department of Biological Science; Nara Women's University; Nara; Japan; c; d; Dep, Hamamatsu University School of Medicine, Journal of Insect Physiology, 49(234), (2003)
Link To Paper

Abstract:
The present study was designed to investigate the process of acidification of yolk granules during embryogenesis. In oocytes of mature Bombyx mori silkmoth yolk proteins and a cysteine protease (pro-form BCP) were found in yolk granules. BCP was localize

Keywords:
Bombyx acid cysteine protease; yolk protein; lysosomal acidification; embryonic development

Materials & Methods:
Oocytes and eggs were broken with fine tungsten needles in buffer A containing 0.01% acridine orange. They were placed in poly-Lysine-coated glass-bottom dishes (MatTek Corp. Ashland MA) and incubated for 5–10 min at room temperature in the dark.

Microscopic Technique
Confocal Microscopy

Cell Type(s)
Oocytes