Technical Reference #231

231.

Validation Of Confocal Laser Scanning Microscopy For Detecting Intracellular Calcium Heterogeneity In Liver Slices Yoneyama; K.; Showa University Health Service Center; Showa University; Tokyo; Japan. J. of Pharmacological and Toxicological Methods; 45; (); 87-9; (00), Showa University, Journal of Pharmacological and Toxicological Methods, 42(231), (2001)
Link To Paper

Abstract:
To investigate changes in the intracellular Ca2+ ([Ca2+]i) in liver lobules under aerobic and hypoxic conditions we measured [Ca2+ ]i in liver slices using a confocal laser scanning microscope (CLSM). The liver lobule is divided into 3 equal parts betwee

Keywords:
Confocal laser scanning microscope; Hypoxia; Intracellular calcium; Lobular heterogeneity; Rat liver slices

Materials & Methods:
After exchanging the medium to glucose-free modified Hanks solution without FCS the liver slices were placed on plastic dishes (MatTEK Co. Ashland MA USA) in a PDMI-2 culture apparatus (Medical Systems Co. NY USA).

Microscopic Technique
Confocal Microscopy

Cell Type(s)
Hepatocytes