Technical Reference #230

230.

Oxidative Stress Increases Internal Calcium Stores And Reduces A Key Mitochondrial Enzyme Gibson; G.E.; Zhang; H.; Xu; H.; Park; L.; and Jeitner T. M.; Cornell University Medical College at Burke Medical Research Institute; White Plains; NY; USA. Biochimica et Biophysica Acta (BBA)/Molecular Basis of Disease; 586; (); 77-89; (00), Burke Medical Research Institute, Biochimica et Biophysica Acta, 1586(230), (2002)
Link To Paper

Abstract:
Cornell University Medical College at Burke Medical Research Institute 785 Mamaroneck Avenue White Plains NY 10605 USA. ggibson@med.cornell.eduFibroblasts from patients with genetic and non-genetic forms of Alzheimer's disease (AD) show many abnor

Keywords:
Alzheimer’s disease; Calcium; Reactive oxygen species; Fibroblast; a-Ketoglutarate dehydrogenase complex; Antioxidant

Materials & Methods:
Cells were sub-cultured weekly by trypsinizing and reseeding at 104/cm2. For imaging experiments fibroblasts were seeded at 500/cm2 on MatTek glass bottom Petri dishes (Ashland MA USA) 5–6 days before the experiments.

Microscopic Technique
Fluorescence Microscopy

Cell Type(s)
A549