Technical Reference #229

Glass Bottom Culture Dishes

This study used MatTek product(s):

Citation in paper containing MatTek reference:
Cover glass-bottomed culture dishes coated with poly-D-lysine (#P35GC-0-14-C) were from MatTek (Ashland; MA)

229.

Effects Of Human Antithymocyte Globulin On Acetylcholine Synthesis; Its Release And Choline Acetyltransferase Transcription In A Human Leukemic T-Cell Line Fujii; T.; Ushiyama; N.; Hosonuma; K.; Suenaga A.; and Kawashima K.; Department of Pharmacology; Kyoritsu College of Pharmacy; Tokyo; Japan. Journal of Neuroimmunology; 8; (-); -8; (00), Kyoritsu College of Pharmacy, Journal of Neuroimmunology, 128(229), (2002)
Link To Paper

Abstract:
In human skin both resident and transiently residing cells are part of the extra- or non-neuronal cholinergic system creating a highly complex and interconnected cosmos in which acetylcholine (ACh) and choline are the natural ligands of nicotinic and mus

Keywords:
Acetylcholine; Choline acetyltransferase; T-lymphocyte

Materials & Methods:
Cover glass-bottomed culture dishes coated with poly-D-lysine (#P35GC-0-14-C) were from MatTek (Ashland MA). AND Once loaded with fluo-3 the cells were diluted 1:9 with RPMI 1640 incubated for an additional 30 min washed with Tyrode-HEPES buffer (20

Microscopic Technique
Confocal Microscopy

Cell Type(s)
CEM