Technical Reference #228

228.

Sustained Ca2+-Induced Ca2+-Release Underlies The Post-Glutamate Lethal Ca2+ Plateau In Older Cultured Hippocampal Neurons Clodfelter; G.V.; Porter; N.M.; Landfield; P.W.; and Thibault; O.; Department of Molecular and Biomedical Pharmacology; University of Kentucky Medical Center; Lexington; KY; USA. European Journal of Pharmacology; 447; (-); 89-00; (00), University of Kentucky, European Journal of Pharmacology, 447(228), (2002)

Abstract:
Several studies have shown that a prolonged Ca2+ elevation follows a glutamate-mediated excitotoxic insult in cultured neurons and may be associated with impending cell death. Recently we showed that the prolonged Ca2+ elevation that emerges as neurons

Keywords:
Endothelin; Nonselective cation channel; G-protein; C6 glioma

Materials & Methods:
The cell suspension was diluted with MEM to a final concentration of 3–5×105 cells/ml and 1 ml added to poly-d-lysine-coated (1 mg/ml) glass-bottom plastic culture dishes (35 mm; Mattek) which contained 1 ml MEM with 10% fetal bovine and 10% horse sera (p

Microscopic Technique
Confocal Microscopy

Cell Type(s)
Neurons - Hippocampal