Technical Reference #227

227.

Group I Metabotropic Glutamate Receptor Inhibition Selectively Blocks A Prolonged Ca2+ Elevation Associated With Age-Dependent Excitotoxicity Attuccib;; S.; Clodfeltera; ;G. V.; Thibaulta; O.; Statona; J.; Moronib; F.; Landfielda ; P. W. ; and Porter a; N. M.; a Department of Molecular and Biomedical Pharmacology; University of Kentucky College of Medicine; Lexington; KY; USA. b Departi, University of Kentucky, Purinergic Signalling, 112(227), (2002)
Link To Paper

Abstract:
The aim of the present paper was to examine in a comparative way the occurrence and the mechanisms of the interactions between adenosine A2A receptors (A2ARs) and metabotropic glutamate 5 receptors (mGlu5Rs) in the hippocampus and the striatum. In rat h

Keywords:
hippocampus; cell culture; aging; NMDA; AIDA; neurotoxicity

Materials & Methods:
For confocal imaging studies the cell suspension was diluted with MEM to a final concentration of ~5×105 cells/ml and 1 ml added to poly-L-lysine-coated (1 mg/ml) glass-bottom culture dishes (35 mm; MatTek Ashland MA USA) containing 1 ml MEM with 10%

Microscopic Technique
Confocal Microscopy

Cell Type(s)
Cerebellar