Technical Reference #226

Glass Bottom Culture Dishes

This study used MatTek product(s):

Citation in paper containing MatTek reference:
Glass-bottomed microwell dishes (35 mm, coated with poly-d-lysine) were from MatTek Corporation (Ashland, MA, USA)

226.

5-Hydroxyindole Potentiates Human A 7 Nicotinic Receptor-Mediated Responses And Enhances Acetylcholine-Induced Glutamate Release In Cerebellar Slices Zwart, R., De Filippi, G., Broad, L.M., McPhie, G.I., Pearson, K.H., Baldwinson T., and Sher E. Eli Lilly and Company Limited, Lilly Research Centre, Surrey, United Kingdom. Neuropharmacology, 4, (), 74-84 (00), Lilly Research, Neuropharmacology, 43(3), 375, (2002)
Link To Paper

Abstract:
The effects of 5-hydroxyindole (5-HI) have been investigated on human 7 nicotinic acetylcholine receptors (nAChRs) expressed in Xenopus oocytes and GH4 cells, on native 7 nAChRs expressed by IMR-32 cells and on 7 nAChR-mediated events in mossy fibre–granu

Keywords:
a7 Nicotinic receptor; Xenopus oocyte; Cerebellar slice; 5-Hydroxyindole; Potentiation; Modulation

Materials & Methods:
Glass-bottomed microwell dishes (35 mm, coated with poly- -lysine) were from MatTek Corporation (Ashland, MA, USA). … For imaging studies, cells were plated on to 35 mm poly- -lysine coated glass-bottomed microwell dishes 24–72 h before recording.

Microscopic Technique
Fluorescence Microscopy, Inverted Epifluorescence Microscopy

Cell Type(s)
GH4