Technical Reference #226

Glass Bottom Culture Dishes

This study used MatTek product(s):

Citation in paper containing MatTek reference:
Glass-bottomed microwell dishes (35 mm; coated with poly-d-lysine) were from MatTek Corporation (Ashland; MA; USA)

226.

5-Hydroxyindole Potentiates Human A 7 Nicotinic Receptor-Mediated Responses And Enhances Acetylcholine-Induced Glutamate Release In Cerebellar Slices Zwart; R.; De Filippi; G.; Broad; L.M.; McPhie; G.I.; Pearson; K.H.; Baldwinson T.; and Sher E. Eli Lilly and Company Limited; Lilly Research Centre; Surrey; United Kingdom. Neuropharmacology; 4; (); 74-84 (00), Lilly Research, Neuropharmacology, 43(226), (2002)
Link To Paper

Abstract:
The effects of 5-hydroxyindole (5-HI) have been investigated on human 7 nicotinic acetylcholine receptors (nAChRs) expressed in Xenopus oocytes and GH4 cells on native 7 nAChRs expressed by IMR-32 cells and on 7 nAChR-mediated events in mossy fibre–granu

Keywords:
a7 Nicotinic receptor; Xenopus oocyte; Cerebellar slice; 5-Hydroxyindole; Potentiation; Modulation

Materials & Methods:
Glass-bottomed microwell dishes (35 mm coated with poly- -lysine) were from MatTek Corporation (Ashland MA USA). … For imaging studies cells were plated on to 35 mm poly- -lysine coated glass-bottomed microwell dishes 24–72 h before recording.

Microscopic Technique
Fluorescence Microscopy, Inverted Epifluorescence Microscopy

Cell Type(s)
GH4