Technical Reference #225

Glass Bottom Culture Dishes

This study used MatTek product(s):

Citation in paper containing MatTek reference:
3.5-cm glass bottom petri dishes (Mattek; Ashland; MA)

225.

Mobile Foci Of Sp100 Do Not Contain Pml: Pml Bodies Are Immobile But Pml And Sp100 Proteins Are Not Wiesmeijer; K.; Molenaar; C.; Bekeer; I.; Tanke H.; and Dirks R.; Department of Molecular Cell Biology; Leiden University Medical Center; Leiden; The Netherlands. Journal of Structural Biology; 40; (-); 80-88; (00), Leiden University, Journal of Structural Biology, 140(225), (2002)

Abstract:
PML bodies are nuclear organelles that are associated with various diseases and are suggested to be involved in multiple cellular activities including transcriptional regulation apoptosis and antiviral defence. Because many proteins with different funct

Keywords:
PML; Sp100; CBP; Time-lapse imaging; FRAP

Materials & Methods:
"VH10 human primary fibroblasts and U2OS human osteosarcoma cells were cultured on 3.5-cm glass bottom petri dishes (Mattek Ashland MA) …"

Microscopic Technique
Fluorescence Microscopy, Fluorescence Redistribution After Photobleaching

Cell Type(s)
U2OS