Technical Reference #223

223.

Spectral Imaging And Linear Un-Mixing Enables Improved Fret Efficiency With A Novel Gfp2–Yfp Fret Pair Zimmermann T; Rietdorf J; Girod A; Georget V; Pepperkok R. Advanced Light Microscopy Facility; European Molecular Biology Laboratory; Meyerhofstr. ; Heidelberg; Germany., European Molecular Biology Laboratory, FEBS Letters, 531(223), (2002)
Link To Paper

Abstract:
Spectral variants of the green fluorescent protein (GFP) have been extensively used as reporters to image molecular interactions in living cells by fluorescence resonance energy transfer (FRET). However those GFP variants which are the most efficient don

Keywords:
Fluorescence resonance energy transfer; Green fluorescent protein; Protein–protein interaction

Materials & Methods:
For expression in mammalian cells HeLa cells were grown on glass bottom dishes (3.5 cm MatTek Ashland MD USA).

Microscopic Technique
Fluorescence Microscopy, Fluorescence Resonance Energy Transfer (FRET)

Cell Type(s)
HeLa