Technical Reference #222

222.

Photoliberating Inositol-1;4;5-Trisphosphate Triggers Atp Release That Is Blocked By The Connexin Mimetic Peptide Gap 26 Katleen Braet a; Wouter Vandammea; Patricia E.M. Martin b; W. Howard Evans b; Luc Leybaert, Ghent University, Cell Calcium, 5(222), (2007)
Link To Paper

Abstract:
Calcium signals can be communicated between cells by the diffusion of a second messenger through gap junction channels or by the release of an extracellular purinergic messenger. We investigated the contribution of these two pathways in endothelial cell l

Keywords:
ATP release; Inositol trisphosphate; Calcium signalling; Flash photolysis; Gap junctions; Hemichannels

Materials & Methods:
Cells were grown (except for immunoblotting) on glass bottom microwells (MatTek Corporation Ashwood MA USA) coated with collagen type I (Boehringer Mannheim Brussels Belgium) and used for experiments upon confluency.

Microscopic Technique
Fluorescence Microscopy

Cell Type(s)
ECV304