Technical Reference #209

209.

Peroxisomes Are Formed from Complex Membrane Structures in PEX6-deficient CHO Cells upon Genetic Complementation Noriyo Hashiguchi; Tomoko Kojidani; Tsuneo Imanaka; Tokuko Haraguchi; Yasushi Hiraoka; Eveline Baumgart; Sadaki Yokota; Toshiro Tsukamoto; and Takashi Osumi, Himeji Insitute of Technology, Molecular Biology of the Cell, 13(209), (2002)
Link To Paper

Abstract:
Pex6p belongs to the AAA family of ATPases. Its CHO mutant ZP92 lacks normal peroxisomes but contains peroxisomal membrane remnants so called peroxisomal ghosts which are detected withanti–70-kDa peroxisomal membrane protein (PMP70) antibody. No per

Materials & Methods:
phGFP(105)-C1 (pGFP) was used as a GFP expression vector (Yamasaki et al. 1998). pGFPSKL was constructed by insertion of a 108-base pair HaeIII/KpnI fragment of the rat acyl-CoA oxidase cDNA encoding a peptide containing a Ser-Lys-Leu-COOH tripeptide (SKL; Miyazawa et al. 1987) between the blunt-ended EcoRI and KpnI sites of pGFP. The rat PEX6 cDNA expression plasmid was pUcD2 92A (Tsukamoto et al. 1995). Rat catalase cDNA was

Microscopic Technique
Fluorescence Microscopy, EpiFluorescence

Cell Type(s)
CHO