Technical Reference #207
Glass Bottom Culture Dishes
This study used MatTek product(s):
Citation in paper containing MatTek reference:
poly-D-lysine-coated coverslips; which were photoetched with an a-numeric grid (Bellco Glass; Inc.; Vineland; NJ); preglued to 35 mm tissue culture dishes (MatTek Corporation; Ashland; MA) 
207. |
GABA Expression Dominates Neuronal Lineage Progression in the Embryonic Rat Neocortex and Facilitates Neurite Outgrowth via GABAa Autoreceptor/Cl- Channels
Dragan Maric; Qi-Ying Liu; Irina Maric; Sabeen Chaudry; Yoong-Hee Chang; Susan V. Smith;
Werner Sieghart; Jean-Marc Fritschy; and Jeffery L. Barker,
National Institute of Health,
The The Journal of Neuroscience,
21(207),
(2001)
Link To Paper
Abstract:
GABA emerges as a trophic signal during rat neocortical development in which it modulates proliferation of neuronal progenitors in the ventricular/subventricular zone (VZ/SVZ) and mediates radial migration of neurons from the VZ/SVZ to the cortical plate/ Keywords:
embryonic; rat; development; cortical; neuritogenesis; GABA; GAD; FACS Materials & Methods:
Experiments were performed on embryos recovered from timedpregnant
Sprague Dawley rats (Taconic Germantown NY) during the
last half of gestation. The embryonic age was determined by comparing
the crown–rump lengths of embryos with previously published values
(Hebel and Stromberg 1986). The day of conception was taken as
embryonic day 1 (E1). All of the research was performed in compliance
with the Animal Welfare Act and the Public Health Service policy on
Humane Care and Use of Laboratory Animals and was approved by the
National Institute of Neurological Disorders and Stroke Animal Care
and Use Committee. Microscopic Technique
Electron Microscopy, immunoelectron Microscopy, Dual Video Microscopy, Light microscopy Cell Type(s)
neocortical |
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