Technical Reference #206

206.

Brief Treatments with Forskolin Enhance S-Phase Entry in Balance Epithelia from the Ears of Rats Mireille Montcouquiol and Jeffrey T. Corwin, University of Virginia School of Medicine, The The Journal of Neuroscience, 21(206), (2001)
Link To Paper

Abstract:
In the ears of mammals hair cell loss results in permanent hearing and balance deficits whereas in fish amphibians and birds the production of replacement hair cells can restore those modalities. In avian ears continuous exposures to forskolin trigg

Keywords:
regeneration; hair cells; cell proliferation; hearing; cAMP; receptor recycling

Materials & Methods:
Epithelial cell cultures. Experiments were conducted in accordance with an approved animal use protocol that adhered to practices in the National Institutes of Health Guide for the Care and Use of Laboratory Animals under the supervision of the University of Virginia Animal Care Advisory Committee. The study used 92 2-d-old (P2) Sprague Dawley rats that were anesthetized with carbon dioxide and decapitated. Heads were skinned and placed in ice-cold 70% ethanol for 10 min. The vestibular

Microscopic Technique
Fluorescence Microscopy, Epifluorsescence, Differential Interference Contrast Microscopy

Cell Type(s)
Mammalian Utricle, Schwann Cells, Retinal gangloin cell