Technical Reference #1950

1950.

Characterization of Cytoplasmic Caspase-2 Activation by Induced Proximity Lisa Bouchier-Hayes; Andrew Oberst; Gavin P. McStay; Samuel Connell; Stephen W.G. Tait; Christopher P. Dillion; Jonathan M. Flanagan; Helen M. Beere; and Douglas R. Green, St. Jude Children's Research Hospital, Molecular Cell, 35(1950), (2009)
Link To Paper

Materials & Methods:
Microscopy Cells were imaged by using a spinning disk confocal microscope (Zeiss). HeLa cells were plated on dishes containing coverslips (Mattek) 24 hr prior to treatment. For time-lapse experiments media on the cells was supplemented with HEPES (20 mM) and 2-mercaptoethanol (55 mM). Cells were allowed to equilibrate to 37 C in 5% CO2 prior to focusing on the cells. Each experiment included control images of untreated cells to ensure that the imaging conditions did not induce phototoxicity and that cell division proceeded.

Microscopic Technique
Confocal Microscopy

Cell Type(s)
HeLa cells