Technical Reference #1933

1933.

Decreased cofilin1 expression is important for compaction during early mouse embryo development Minyue Ma; Lin Zhou; Xuejiang Guo; Zhou Lv; Yang Yu; Chenhui Ding; Ping Zhang; Ye Bi; Jin Xie; Liu Wang; Min Lin; Zuomin Zhou; Ran Huo; Jiahao Sha; Qi Zhou, Nanjing Medical University, Biochimica et Biophysica Acta, 1793(1933), (2009)
Link To Paper

Keywords:
Compaction; Polarization; Early development; Embryo; Cofilin1

Materials & Methods:
Two-cell stage embryos were flushed from the female reproductive tract. One of the two blastomeres was injected with an anticofilin1 antibody mixed with FITC-dextran (Sigma) and was used for tracing. The embryos were grown on 35 mm Glass Bottom Dishes (MatTek CatNo. P35G-1.0-14-C). As compaction began images of development were acquired automatically using a Nikon TE2000E inverted microscope fitted with a ×10 Nikon Plan Fluor objective a Proscan II stage (Proscan Prior) and a Nikon CCD camera. Fluorescence and differential interference contrast images were obtained every 15 min during compaction. The microscope was housed in a custom-designed 37°C chamber with humidified 5% CO2.

Microscopic Technique
Inverted Microscopy, Fluorescence Microscopy

Cell Type(s)
Embryos